DNA replication – Speck Lab / DNA Replication Group

09Jul20219 July 2021

VACANCY: LMS 3.5-year Studentship

Project Title: Development of high throughput assay for screening of novel DNA replication inhibitors for therapeutic purposes Supervisors: Professor Christian Speck, Professor David Rueda Funding: Tuition fees plus £21,000 pa stipend for 3.5 years Date posted: 09 July 2021 Closing date: 04 August 2021 The student will develop a fluorescence-based assay to identify novel DNA replication inhibitors for anti-cancer therapy. Inhibitors will be consequently characterised for their impact on the multi-step DNA replication process and on cancer cell growth. This interdisciplinary project will train the student in biochemistry, biophysics and drug screening. Project details | LMS 3.5-year Studentships | Apply

By SpeckLabLab Group, Research, StudentshipsDNA replication, drug progression, drug screening, DNA replication inhibitors, fluorescence-based assay, protein biochemistry0 CommentsRead more...

23Jun202123 June 2021

New paper on the molecular mechanism in the licensing of eukaryotic replication origins

Feng, X., Noguchi, Y., Barbon, M., Stillman, B., Speck, C., Li, H. (2021). The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6. Nature Communications 12, 3883. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchCdc6, DNA replication, cryo-EM, ORC-Cdc6, ORC activation0 CommentsRead more...

15Jul202015 July 2020

New paper on the structural mechanism of helicase loading

Yuan, Z., Schneider, S., Dodd, T., Riera, A., Bai, L., Yan, C., Magdalou, I., Ivanov, I., Stillman, B., Li, H., Speck, C. (2020). Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6. Proceedings of the National Academy of Sciences of the United States of America 117, 17747-17756. Abstract | Full Text |

By SpeckLabLab Group, Publications, Researchhelicase loading, DNA replication, origin DNA, cryo-EM, ORC-Cdc60 CommentsRead more...

25Oct201725 October 2017

New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538. Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7, helicase, DNA replication, cryo-EM, DNA unwinding, lagging-strand DNA exclusion model0 CommentsRead more...

01Jun20171 June 2017

New review paper on initiation of DNA replication

Riera, A., Barbon, M., Noguchi, Y., Reuter, L. M., Schneider, S., Speck, C. (2017). From structure to mechanism — understanding initiation of DNA replication. Genes & Development 31, 1073-1078. Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchDNA, eukaryotes, MCM2-7, structure, DNA replication, replication fork0 CommentsRead more...

17Jul201617 July 2016

New article commentary on Cdc45 overexpression

Speck, C. (2016). Exceeding the limits – Cdc45 overexpression turns bad. Cell Cycle 15, 1809-1810.
Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7, DNA replication, Cdc45, Cdc45 overexpression, apoptosis, CMG helicase, genome stability, replication origin firing, RPA, replication fork, single stranded DNA0 CommentsRead more...

27May201627 May 2016

New article about replicating repetitive DNA

Tognetti, S., Speck, C. (2016). Replicating repetitive DNA. Nature Cell Biology 18, 593-594.
Abstract |

By SpeckLabLab Group, Publications, ResearchDNA replication, repetitive DNA0 CommentsRead more...

25Aug201525 August 2015

New paper shows Cdc6 ATPase activity promotes DNA replication

Chang, F., Riera, A., Evrin, C., Sun, J., Li, H., Speck, C., Weinreich, M. (2015). Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication. eLife 4, e05759.
Abstract | Full text | Biomedical Picture of the Day | MRC Clinical Sciences Centre News | Imperial College London News |

By SpeckLabLab Group, Publications, ResearchCdc6, ORC, DNA replication, AAA+, ATPase, helicase loader0 CommentsRead more...

20Jan201520 January 2015

New review paper on the role of MCM2-7 in DNA replication

Riera, A., Speck, C. (2015). MCM2-7 – Opening the gate to DNA Replication. Cell Cycle 14, 6-8.
Full text |

By SpeckLabLab Group, Publications, ResearchDNA, MCM2-7, pre-RC, replicative helicase, DNA replication, DNA entry gate, DNA licensing0 CommentsRead more...

16Sep201416 September 2014

Silvia Tognetti successfully defends her PhD thesis

Silvia Tognetti successfully defends her PhD thesis, on helicase activation mechanisms during initiation of DNA replication. Congratulations Dr Tognetti!

By SpeckLabLab Group, People, ResearchDNA, Silvia Tognetti, helicase activation, DNA replication0 CommentsRead more...

01Aug20141 August 2014

New paper on regulation of loading of MCM2-7 onto DNA

Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
Abstract | Imperial College London News |

By SpeckLabLab Group, Publications, ResearchMCM2-7, pre-RC, replicative helicase, DNA replication, DNA licensing, genomic stability0 CommentsRead more...

21Jun201421 June 2014

New paper on the formation of the MCM2-7 double-hexamer

Riera, A., Tognetti, S., Speck, C. (2014). Helicase loading: How to build a MCM2-7 double-hexamer. Seminars in Cell & Developmental Biology 30C, 104-109.
Abstract |

By SpeckLabLab Group, Publications, ResearchMCM2-7, pre-RC, helicase loading, DNA replication, DNA licensing0 CommentsRead more...

Tag - DNA replication