DNA replication – Speck Lab / DNA Replication Group

21Sep202121 September 2021

VACANCY: Research associate x 2 in biochemistry and structural biology (MED02737)

Reference: MED02737 Date posted: 21 September 2021 Closing date: 19 October 2021 Two full-time research associate positions are available to investigate mechanisms of human DNA replication and their misregulation in cancer employing biochemical and structural or genomic approaches, with the aim of discovering how DNA replication origins are specified (position 1), and how DNA replication is regulated (position 2), to support genome stability and achieve faithful DNA replication. Position 1 requires expertise in biochemistry, while position 2 in cryo-EM. Job details | Apply

By SpeckLabFellowships, Lab Group, Research, VacanciesDNA replication, cryo-EM, postdoctoral position, biochemistry, genomics, proteomics, DNA misregulation, DNA regulation, DNA replication origins0 CommentsRead more...

21Sep202121 September 2021

VACANCY: Research associate in genomics (MED02738)

Reference: MED02738 Date posted: 21 September 2021 Closing date: 19 October 2021 A full-time research associate is available to investigate mechanisms of human DNA replication and their misregulation in cancer employing biochemical and structural or genomic approaches. The successful applicant will employ high resolution genomics (ChIP-Exo or CUT&RUN), proteomic (ChIP-MS) and cell biological approaches to discover novel mechanisms in the function of human DNA replication origins and explore how misregulation of replication origins leads to genomic instability. Expertise in high-resolution genomics and bioinformatic analysis of data is essential. Job details | Apply

By SpeckLabFellowships, Lab Group, Research, VacanciesDNA replication, postdoctoral position, genomics, DNA misregulation, DNA regulation, genomic instability0 CommentsRead more...

23Jun202123 June 2021

New paper on the molecular mechanism in the licensing of eukaryotic replication origins

Feng, X., Noguchi, Y., Barbon, M., Stillman, B., Speck, C., Li, H. (2021). The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6. Nature Communications 12, 3883. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchCdc6, DNA replication, cryo-EM, ORC-Cdc6, ORC activation0 CommentsRead more...

15Jul202015 July 2020

New paper on the structural mechanism of helicase loading

Yuan, Z., Schneider, S., Dodd, T., Riera, A., Bai, L., Yan, C., Magdalou, I., Ivanov, I., Stillman, B., Li, H., Speck, C. (2020). Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6. Proceedings of the National Academy of Sciences of the United States of America 117, 17747-17756. Abstract | Full Text |

By SpeckLabLab Group, Publications, Researchhelicase loading, DNA replication, origin DNA, cryo-EM, ORC-Cdc60 CommentsRead more...

25Oct201725 October 2017

New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538. Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7, helicase, DNA replication, cryo-EM, DNA unwinding, lagging-strand DNA exclusion model0 CommentsRead more...

01Jun20171 June 2017

New review paper on initiation of DNA replication

Riera, A., Barbon, M., Noguchi, Y., Reuter, L. M., Schneider, S., Speck, C. (2017). From structure to mechanism — understanding initiation of DNA replication. Genes & Development 31, 1073-1078. Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchDNA, eukaryotes, MCM2-7, structure, DNA replication, replication fork0 CommentsRead more...

17Jul201617 July 2016

New article commentary on Cdc45 overexpression

Speck, C. (2016). Exceeding the limits – Cdc45 overexpression turns bad. Cell Cycle 15, 1809-1810.
Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7, DNA replication, Cdc45, Cdc45 overexpression, apoptosis, CMG helicase, genome stability, replication origin firing, RPA, replication fork, single stranded DNA0 CommentsRead more...

27May201627 May 2016

New article about replicating repetitive DNA

Tognetti, S., Speck, C. (2016). Replicating repetitive DNA. Nature Cell Biology 18, 593-594.
Abstract |

By SpeckLabLab Group, Publications, ResearchDNA replication, repetitive DNA0 CommentsRead more...

25Aug201525 August 2015

New paper shows Cdc6 ATPase activity promotes DNA replication

Chang, F., Riera, A., Evrin, C., Sun, J., Li, H., Speck, C., Weinreich, M. (2015). Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication. eLife 4, e05759.
Abstract | Full text | Biomedical Picture of the Day | MRC Clinical Sciences Centre News | Imperial College London News |

By SpeckLabLab Group, Publications, ResearchCdc6, ORC, DNA replication, AAA+, ATPase, helicase loader0 CommentsRead more...

20Jan201520 January 2015

New review paper on the role of MCM2-7 in DNA replication

Riera, A., Speck, C. (2015). MCM2-7 – Opening the gate to DNA Replication. Cell Cycle 14, 6-8.
Full text |

By SpeckLabLab Group, Publications, ResearchDNA, MCM2-7, pre-RC, replicative helicase, DNA replication, DNA entry gate, DNA licensing0 CommentsRead more...

16Sep201416 September 2014

Silvia Tognetti successfully defends her PhD thesis

Silvia Tognetti successfully defends her PhD thesis, on helicase activation mechanisms during initiation of DNA replication. Congratulations Dr Tognetti!

By SpeckLabLab Group, People, ResearchDNA, Silvia Tognetti, helicase activation, DNA replication0 CommentsRead more...

01Aug20141 August 2014

New paper on regulation of loading of MCM2-7 onto DNA

Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
Abstract | Imperial College London News |

By SpeckLabLab Group, Publications, ResearchMCM2-7, pre-RC, replicative helicase, DNA replication, DNA licensing, genomic stability0 CommentsRead more...

Tag - DNA replication