DNA replication – Speck Lab / DNA Replication Group

25May202225 May 2022

New paper on DDK structure, control and selective activation of the MCM2-7 helicase

Saleh, A., Noguchi, Y., Aramayo, R., Ivanova, M. E., Stevens, K. M., Montoya, A., Sunidhi, S., Carranza, N. L., Skwark, M. J., Speck, C. (2022). The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer. Nature Communication 13:2915. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchDNA replication, Cdc7, Cdc7-Dbf4, Cdc7-Dbf4 kinase, Dbf4, DDK, MCM2-7 phosphorylation, MCM2-70 CommentsRead more...

23Jun202123 June 2021

New paper on the molecular mechanism in the licensing of eukaryotic replication origins

Feng, X., Noguchi, Y., Barbon, M., Stillman, B., Speck, C., Li, H. (2021). The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6. Nature Communications 12:3883. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchORC-Cdc6, ORC activation, Cdc6, DNA replication, cryo-EM0 CommentsRead more...

14Oct202014 October 2020

New paper on evolution of DNA replication origin and gene silencing

Hu, Y., Tareen, A., Sheu, Y. J., Ireland, W.T ., Speck, C., Li, H., Joshua-Tor, L., Kinney, J. B., Stillman, B. (2020). Evolution of DNA replication origin specification and gene silencing mechanisms. Nature Communications 11:5175. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchDNA replication, DNA replication origin, Evolution, Gene silencing0 CommentsRead more...

15Jul202015 July 2020

New paper on the structural mechanism of helicase loading

Yuan, Z., Schneider, S., Dodd, T., Riera, A., Bai, L., Yan, C., Magdalou, I., Ivanov, I., Stillman, B., Li, H., Speck, C. (2020). Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6. Proceedings of the National Academy of Sciences of the United States of America 117, 17747-17756. Abstract | Full Text |

By SpeckLabLab Group, Publications, Researchhelicase loading, DNA replication, origin DNA, cryo-EM, ORC-Cdc60 CommentsRead more...

25Oct201725 October 2017

New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538. Abstract | Full text |

By SpeckLabLab Group, Publications, Researchhelicase, DNA replication, cryo-EM, DNA unwinding, lagging-strand DNA exclusion model, MCM2-70 CommentsRead more...

01Jun20171 June 2017

New review paper on initiation of DNA replication

Riera, A., Barbon, M., Noguchi, Y., Reuter, L. M., Schneider, S., Speck, C. (2017). From structure to mechanism — understanding initiation of DNA replication. Genes & Development 31, 1073-1078. Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchDNA, eukaryotes, MCM2-7, structure, DNA replication, replication fork0 CommentsRead more...

17Jul201617 July 2016

New article commentary on Cdc45 overexpression

Speck, C. (2016). Exceeding the limits – Cdc45 overexpression turns bad. Cell Cycle 15, 1809-1810.
Full text |

By SpeckLabResearch, Lab Group, PublicationsDNA replication, Cdc45, Cdc45 overexpression, apoptosis, CMG helicase, genome stability, replication origin firing, MCM2-7, RPA, replication fork, single stranded DNA0 CommentsRead more...

27May201627 May 2016

New article about replicating repetitive DNA

Tognetti, S., Speck, C. (2016). Replicating repetitive DNA. Nature Cell Biology 18, 593-594.
Abstract |

By SpeckLabLab Group, Publications, ResearchDNA replication, repetitive DNA0 CommentsRead more...

25Aug201525 August 2015

New paper shows Cdc6 ATPase activity promotes DNA replication

Chang, F., Riera, A., Evrin, C., Sun, J., Li, H., Speck, C., Weinreich, M. (2015). Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication. eLife 4, e05759.
Abstract | Full text | Biomedical Picture of the Day | MRC Clinical Sciences Centre News | Imperial College London News |

By SpeckLabLab Group, Publications, ResearchAAA+, ATPase, helicase loader, Cdc6, ORC, DNA replication0 CommentsRead more...

20Jan201520 January 2015

New review paper on the role of MCM2-7 in DNA replication

Riera, A., Speck, C. (2015). MCM2-7 – Opening the gate to DNA Replication. Cell Cycle 14, 6-8.
Full text |

By SpeckLabLab Group, Publications, Researchreplicative helicase, DNA replication, DNA entry gate, DNA licensing, DNA, MCM2-7, pre-RC0 CommentsRead more...

16Sep201416 September 2014

Silvia Tognetti successfully defends her PhD thesis

Silvia Tognetti successfully defends her PhD thesis, on helicase activation mechanisms during initiation of DNA replication. Congratulations Dr Tognetti!

By SpeckLabPeople, Research, Lab GroupSilvia Tognetti, helicase activation, DNA replication, DNA0 CommentsRead more...

01Aug20141 August 2014

New paper on regulation of loading of MCM2-7 onto DNA

Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
Abstract | Imperial College London News |

By SpeckLabLab Group, Publications, ResearchMCM2-7, pre-RC, replicative helicase, DNA replication, DNA licensing, genome stability0 CommentsRead more...

Tag - DNA replication