Category: Publications

24Aug202424 August 2024

New paper on genome-wide origin licensing

Reuter L. M., Khadayate, S. P., Mossler, A., Liebl, K., Faull, S. V., Karimi, M. M., Speck, C. (2024). MCM2-7 loading-dependent ORC release ensures genome-wide origin licensing. Nature Communications 15: 7306. Full text |

By SpeckLabResearch, Publications, Lab GroupDNA licensing, MCM2-7, ORCRead more...

04Sep20234 September 2023

New review paper on pre-RNA splicing in metabolic homeostasis and liver disease

Jobbins, A. M., Yu, S., Paterson, H. A. B., Maude, H., Kefala-Stavridi, A., Speck, C., Cebola, I., Vernia, S. (2023). Pre-RNA splicing in metabolic homeostasis and liver disease. Trends in Endocrinology & Metabolism 34, P823-P837. Abstract | Full text |

By SpeckLabPublications, Research, Lab Groupreview, pre-mRNA splicing, metabolic homeostasis, liver, alternative RNA splicingRead more...

25May202225 May 2022

New paper on DDK structure, control and selective activation of the MCM2-7 helicase

Saleh, A., Noguchi, Y., Aramayo, R., Ivanova, M. E., Stevens, K. M., Montoya, A., Sunidhi, S., Carranza, N. L., Skwark, M. J., Speck, C. (2022). The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer. Nature Communication 13:2915. Abstract | Full Text |

By SpeckLabResearch, Publications, Lab GroupMCM2-7, MCM2-7 phosphorylation, DDK, Dbf4, Cdc7-Dbf4 kinase, Cdc7-Dbf4, Cdc7, DNA replicationRead more...

23Jun202123 June 2021

New paper on the molecular mechanism in the licensing of eukaryotic replication origins

Feng, X., Noguchi, Y., Barbon, M., Stillman, B., Speck, C., Li, H. (2021). The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6. Nature Communications 12:3883. Abstract | Full Text |

By SpeckLabPublications, Research, Lab GroupORC activation, ORC-Cdc6, cryo-EM, DNA replication, Cdc6Read more...

14Oct202014 October 2020

New paper on evolution of DNA replication origin and gene silencing

Hu, Y., Tareen, A., Sheu, Y. J., Ireland, W.T ., Speck, C., Li, H., Joshua-Tor, L., Kinney, J. B., Stillman, B. (2020). Evolution of DNA replication origin specification and gene silencing mechanisms. Nature Communications 11:5175. Abstract | Full Text |

By SpeckLabResearch, Publications, Lab GroupGene silencing, Evolution, DNA replication origin, DNA replicationRead more...

15Jul202015 July 2020

New paper on the structural mechanism of helicase loading

Yuan, Z., Schneider, S., Dodd, T., Riera, A., Bai, L., Yan, C., Magdalou, I., Ivanov, I., Stillman, B., Li, H., Speck, C. (2020). Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6. Proceedings of the National Academy of Sciences of the United States of America 117, 17747-17756. Abstract | Full Text |

By SpeckLabResearch, Publications, Lab GroupORC-Cdc6, cryo-EM, origin DNA, DNA replication, helicase loadingRead more...

25Oct201725 October 2017

New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538. Abstract | Full text |

By SpeckLabResearch, Publications, Lab Grouplagging-strand DNA exclusion model, DNA unwinding, cryo-EM, DNA replication, helicase, MCM2-7Read more...

01Jun20171 June 2017

New review paper on initiation of DNA replication

Riera, A., Barbon, M., Noguchi, Y., Reuter, L. M., Schneider, S., Speck, C. (2017). From structure to mechanism — understanding initiation of DNA replication. Genes & Development 31, 1073-1078. Abstract | Full text |

By SpeckLabResearch, Publications, Lab Groupstructure, replication fork, DNA replication, MCM2-7, eukaryotes, DNA0 CommentsRead more...

13Feb201713 February 2017

New paper on MCM2-7 replicative helicase loading

Yuan, Z., Riera, A., Bai, L., Sun, J., Nandi, S., Spanos, C., Chen, Z. A., Barbon, M., Rappsilber, J., Stillman, B., Speck, C., Li, H. (2017). Structural basis of Mcm2–7 replicative helicase loading by ORC–Cdc6 and Cdt1. Nature Structure & Molecular Biology 24, 316-324.
Abstract | Full text |

By SpeckLabResearch, Publications, Lab GroupMCM2-7 replicative helicase, Cdt1, ORC-Cdc6, MCM2-7 loadingRead more...

17Jul201617 July 2016

New article commentary on Cdc45 overexpression

Speck, C. (2016). Exceeding the limits – Cdc45 overexpression turns bad. Cell Cycle 15, 1809-1810.
Full text |

By SpeckLabResearch, Publications, Lab GroupRPA, replication origin firing, genome stability, CMG helicase, apoptosis, Cdc45 overexpression, Cdc45, DNA replication, MCM2-7, replication fork, single stranded DNARead more...

27May201627 May 2016

New article about replicating repetitive DNA

Tognetti, S., Speck, C. (2016). Replicating repetitive DNA. Nature Cell Biology 18, 593-594.
Abstract |

By SpeckLabResearch, Publications, Lab Grouprepetitive DNA, DNA replicationRead more...

03Sep20153 September 2015

New paper on assembly of the eukaryotic replicative helicase

Herrera, M. C.*, Tognetti, S.*, Riera, A., Zech, J., Clarke, P., Fernández-Cid, A., Speck, C. (2015). A reconstituted system reveals how activating and inhibitory interactions control DDK dependent assembly of the eukaryotic replicative helicase. Nucleic Acids Research 43, 10238-10250.
*Shared first authorship
Abstract | Full text |

By SpeckLabResearch, Publications, Lab GroupDbf4-dependent kinase, MCM2-7 double hexamer, helicase activation, pre-RC, MCM2-7Read more...