Publications – Speck Lab / DNA Replication Group

23Jun202123 June 2021

New paper on the molecular mechanism in the licensing of eukaryotic replication origins

Feng, X., Noguchi, Y., Barbon, M., Stillman, B., Speck, C., Li, H. (2021). The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6. Nature Communications 12, 3883. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchCdc6, DNA replication, cryo-EM, ORC-Cdc6, ORC activation0 CommentsRead more...

15Jul202015 July 2020

New paper on the structural mechanism of helicase loading

Yuan, Z., Schneider, S., Dodd, T., Riera, A., Bai, L., Yan, C., Magdalou, I., Ivanov, I., Stillman, B., Li, H., Speck, C. (2020). Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6. Proceedings of the National Academy of Sciences of the United States of America 117, 17747-17756. Abstract | Full Text |

By SpeckLabLab Group, Publications, Researchhelicase loading, DNA replication, origin DNA, cryo-EM, ORC-Cdc60 CommentsRead more...

25Oct201725 October 2017

New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538. Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7, helicase, DNA replication, cryo-EM, DNA unwinding, lagging-strand DNA exclusion model0 CommentsRead more...

01Jun20171 June 2017

New review paper on initiation of DNA replication

Riera, A., Barbon, M., Noguchi, Y., Reuter, L. M., Schneider, S., Speck, C. (2017). From structure to mechanism — understanding initiation of DNA replication. Genes & Development 31, 1073-1078. Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchDNA, eukaryotes, MCM2-7, structure, DNA replication, replication fork0 CommentsRead more...

13Feb201713 February 2017

New paper on MCM2-7 replicative helicase loading

Yuan, Z., Riera, A., Bai, L., Sun, J., Nandi, S., Spanos, C., Chen, Z. A., Barbon, M., Rappsilber, J., Stillman, B., Speck, C., Li, H. (2017). Structural basis of Mcm2–7 replicative helicase loading by ORC–Cdc6 and Cdt1. Nature Structure & Molecular Biology 24, 316-324.
Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7 loading, ORC-Cdc6, Cdt1, MCM2-7 replicative helicase0 CommentsRead more...

17Jul201617 July 2016

New article commentary on Cdc45 overexpression

Speck, C. (2016). Exceeding the limits – Cdc45 overexpression turns bad. Cell Cycle 15, 1809-1810.
Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7, DNA replication, Cdc45, Cdc45 overexpression, apoptosis, CMG helicase, genome stability, replication origin firing, RPA, replication fork, single stranded DNA0 CommentsRead more...

27May201627 May 2016

New article about replicating repetitive DNA

Tognetti, S., Speck, C. (2016). Replicating repetitive DNA. Nature Cell Biology 18, 593-594.
Abstract |

By SpeckLabLab Group, Publications, ResearchDNA replication, repetitive DNA0 CommentsRead more...

03Sep20153 September 2015

New paper on assembly of the eukaryotic replicative helicase

Herrera, M. C.*, Tognetti, S.*, Riera, A., Zech, J., Clarke, P., Fernández-Cid, A., Speck, C. (2015). A reconstituted system reveals how activating and inhibitory interactions control DDK dependent assembly of the eukaryotic replicative helicase. Nucleic Acids Research 43, 10238-10250.
*Shared first authorship
Abstract | Full text |

By SpeckLabLab Group, Publications, ResearchMCM2-7, pre-RC, helicase activation, MCM2-7 double hexamer, Dbf4-dependent kinase0 CommentsRead more...

25Aug201525 August 2015

New paper shows Cdc6 ATPase activity promotes DNA replication

Chang, F., Riera, A., Evrin, C., Sun, J., Li, H., Speck, C., Weinreich, M. (2015). Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication. eLife 4, e05759.
Abstract | Full text | Biomedical Picture of the Day | MRC Clinical Sciences Centre News | Imperial College London News |

By SpeckLabLab Group, Publications, ResearchCdc6, ORC, DNA replication, AAA+, ATPase, helicase loader0 CommentsRead more...

01Mar20151 March 2015

New paper on activation of the eukaryotic replicative MCM2-7 helicase

Tognetti, S., Riera, A., Speck, C. (2015) Switch on the engine: how the eukaryotic replicative MCM2–7 helicase becomes activated. Chromosoma 124, 13-26.
Abstract |