Research – Speck Lab / DNA Replication Group

25May202225 May 2022

New paper on DDK structure, control and selective activation of the MCM2-7 helicase

Saleh, A., Noguchi, Y., Aramayo, R., Ivanova, M. E., Stevens, K. M., Montoya, A., Sunidhi, S., Carranza, N. L., Skwark, M. J., Speck, C. (2022). The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer. Nature Communication 13:2915. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchDNA replication, Cdc7, Cdc7-Dbf4, Cdc7-Dbf4 kinase, Dbf4, DDK, MCM2-7 phosphorylation, MCM2-70 CommentsRead more...

23Jun202123 June 2021

New paper on the molecular mechanism in the licensing of eukaryotic replication origins

Feng, X., Noguchi, Y., Barbon, M., Stillman, B., Speck, C., Li, H. (2021). The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6. Nature Communications 12:3883. Abstract | Full Text |

By SpeckLabLab Group, Publications, Researchcryo-EM, ORC-Cdc6, ORC activation, Cdc6, DNA replication0 CommentsRead more...

14Oct202014 October 2020

New paper on evolution of DNA replication origin and gene silencing

Hu, Y., Tareen, A., Sheu, Y. J., Ireland, W.T ., Speck, C., Li, H., Joshua-Tor, L., Kinney, J. B., Stillman, B. (2020). Evolution of DNA replication origin specification and gene silencing mechanisms. Nature Communications 11:5175. Abstract | Full Text |

By SpeckLabLab Group, Publications, ResearchDNA replication, DNA replication origin, Evolution, Gene silencing0 CommentsRead more...

15Jul202015 July 2020

New paper on the structural mechanism of helicase loading

Yuan, Z., Schneider, S., Dodd, T., Riera, A., Bai, L., Yan, C., Magdalou, I., Ivanov, I., Stillman, B., Li, H., Speck, C. (2020). Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6. Proceedings of the National Academy of Sciences of the United States of America 117, 17747-17756. Abstract | Full Text |

By SpeckLabResearch, Lab Group, Publicationshelicase loading, DNA replication, origin DNA, cryo-EM, ORC-Cdc60 CommentsRead more...

07Nov20197 November 2019

Group awarded BBSRC project grant

The Group's Biotechnology and Biological Sciences Research Council (BBSRC) grant application was successful! Huge congratulations to Sarah Schneider, Almutasem Saleh and Yasunori Noguchi for writing a fantastic proposal and achieving a superb ranking of fourth out of 89. Two new postdoctoral positions will open soon. Interested candidates should contact Christian Speck.

By SpeckLabLab Group, Research, GrantsBBSRC, Biotechnology and Biological Sciences Research Council0 CommentsRead more...

11Oct201911 October 2019

Data collected using new Titan Krios cryo-TEM

First data collected using the London Consortium for cryo-EM's (LonCEM's) new Titan Krios Cryo Transmission Electron Microscope with the Gatan K3™ camera in super-resolution mode. A dream come true.

By SpeckLabResearch, EquipmentLondon consortium for cryo-EM, Titan Krios Cryo Transmission Electron Microscope, cryo-TEM, electron microscopy, cryo-EM, LonCEM0 CommentsRead more...

25Oct201725 October 2017

New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538. Abstract | Full text |

By SpeckLabLab Group, Publications, Researchlagging-strand DNA exclusion model, MCM2-7, helicase, DNA replication, cryo-EM, DNA unwinding0 CommentsRead more...

06Jul20176 July 2017

Katalin Kondas participates in EMBO Practical Course

As a selected participant, Katalin Kondas attends the EMBO Practical Course "High-throughput protein production and crystallization”, 6 – 14 July 2017 in Harwell, Oxfordshire, UK.

By SpeckLabAwards, Research, WorkshopsEMBO Practical Course, Kaitlin Kondas0 CommentsRead more...

01Jun20171 June 2017

New review paper on initiation of DNA replication

Riera, A., Barbon, M., Noguchi, Y., Reuter, L. M., Schneider, S., Speck, C. (2017). From structure to mechanism — understanding initiation of DNA replication. Genes & Development 31, 1073-1078. Abstract | Full text |

By SpeckLabResearch, Lab Group, PublicationsDNA replication, replication fork, DNA, eukaryotes, MCM2-7, structure0 CommentsRead more...

13Feb201713 February 2017

New paper on MCM2-7 replicative helicase loading

Yuan, Z., Riera, A., Bai, L., Sun, J., Nandi, S., Spanos, C., Chen, Z. A., Barbon, M., Rappsilber, J., Stillman, B., Speck, C., Li, H. (2017). Structural basis of Mcm2–7 replicative helicase loading by ORC–Cdc6 and Cdt1. Nature Structure & Molecular Biology 24, 316-324.
Abstract | Full text |