Tag - MCM2-7

New paper on DDK structure, control and selective activation of the MCM2-7 helicase

Saleh, A., Noguchi, Y., Aramayo, R., Ivanova, M. E., Stevens, K. M., Montoya, A., Sunidhi, S., Carranza, N. L., Skwark, M. J., Speck, C. (2022). The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer. Nature Communication 13:2915. Abstract | Full Text |

New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538. Abstract | Full text |

New paper on assembly of the eukaryotic replicative helicase

Herrera, M. C.*, Tognetti, S.*, Riera, A., Zech, J., Clarke, P., Fernández-Cid, A., Speck, C. (2015). A reconstituted system reveals how activating and inhibitory interactions control DDK dependent assembly of the eukaryotic replicative helicase. Nucleic Acids Research 43, 10238-10250.
*Shared first authorship
Abstract | Full text |

New paper on Mcm2–7 double-hexamer assembly and function

Sun, J.*, Fernandez-Cid, A.*, Riera, A.*, Tognetti, S., Yuan, Z., Stillman, B.†, Speck, C.†, Li, H.† (2014). Structural and mechanistic insights into Mcm2–7 double-hexamer assembly and function. Genes & Development 28, 2291-2303.
*Shared first authorship, †Corresponding authors
Abstract | Biomedical Picture of the Day |

New paper on regulation of loading of MCM2-7 onto DNA

Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
Abstract | Imperial College London News |

New paper establishes architecture of the OCCM complex

Sun, J.*, Evrin, C.*, Samel, S. A., Fernández-Cid, A., Riera, A., Kawakami, H., Stillman, B.†, Speck, C.†, Li, H.† (2013). Cryo-EM structure of a helicase loading intermediate containing ORC–Cdc6–Cdt1–MCM2-7 bound to DNA. Nature Structural & Molecular Biology 20, 944-951.
*Shared first authorship; †Corresponding authors
Abstract | Full text | Brookhaven National Laboratory Newsroom |