Tag - DNA licensing

VACANCY: Research associate in molecular machines (MED04213)

Reference: MED04213 Date posted: 06 November 2023 Closing date: 03 January 2024 Applications are invited for a full-time postdoctoral research associate position with the aim of uncovering fundamental and medically relevant mechanisms that guarantee efficient human DNA replication employing cutting-edge biochemical and structural approaches.  The project's primary objective is to discover how 1) DNA replication origins are specified through epigenetic mechanisms and specific recruitment factors and 2) DNA replication is regulated to allow faithful DNA replication.  The successful candidate will be able to take advantage of a recently established reconstituted system, which allows us to address these questions for the first time. Job details | Apply

New paper on regulation of loading of MCM2-7 onto DNA

Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
Abstract | Imperial College London News |

New paper reveals mechanisms redefining the understanding of DNA licensing

Fernández-Cid, A.*, Riera, A.*, Tognetti, S., Herrera, M. C., Samel, S., Evrin, C., Winkler, C., Gardenal, E., Uhle. S., Speck, C. (2013). An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer formation. Molecular Cell 50, 577-588.
*Shared first authorship
Abstract | Full text | Molecular Cell Preview |

New paper on double-hexameric MCM2-7 complex loading onto origin DNA

Evrin, C., Clarke, P., Zech, J., Lurz, R., Sun, J., Uhle, S., Li, H., Stillman, B., Speck, C. (2009). A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA. Proceedings of the National Academy of Sciences of the United States of America 106, 20240-20245.
Abstract | Full text | F1000Prime |