Tag - replicative helicase

VACANCY: Enterprise DTP iCASE Doctoral Training Programme studentship

Project title: Developing advanced mass photometry methods for analysis of the multi-step helicase loading process
Lead supervisor: Christian Speck
Funding: EPSRC
Start date: October 2021
Duration: 3.5 years
Date posted: 12 November 2020
Closing date: 07 January 2021

The student will use and develop a novel single-molecule biophysical method (mass photometry) to investigate a key step in DNA replication, the loading of the replicative helicase on DNA, and explore this multi-step reaction in a time resolved manner. This work will address how DNA replication works and contribute to the development of helicase loading inhibitors as a potential anti-cancer therapy.

The project is a collaboration between Refeyn Ltd and the Imperial College London research groups of Christian Speck and David Rueda.  Refeyn, a company based in Oxford, has recently developed and brought to market a breakthrough technology that measures the molecular weight of individual proteins and protein complexes by mass photometry.  This revolutionary biophysical technique is highly-accurate, very fast, label-free and allows the use of native proteins.

The group of Christian Speck, which includes seven postdocs, three PhD students and two technicians, applies a range of biochemical, structural, proteomic and genetic/genomic approaches in order to investigate DNA replication and chromatin structure.  The Group works in a highly collaborative way and provides PhD students with a multidisciplinary learning environment.  Applicants that interested in genome stability and method development and have a good background in biochemistry or biophysics are strongly encouraged to apply.

Interested candidates can contact Christian Speck directly for more information.

Enterprise DTP: iCASE Doctoral Training Programme | Project details | Apply

New paper on Mcm2–7 double-hexamer assembly and function

Sun, J.*, Fernandez-Cid, A.*, Riera, A.*, Tognetti, S., Yuan, Z., Stillman, B.†, Speck, C.†, Li, H.† (2014). Structural and mechanistic insights into Mcm2–7 double-hexamer assembly and function. Genes & Development 28, 2291-2303.
*Shared first authorship, †Corresponding authors
Abstract | Biomedical Picture of the Day |

New paper on regulation of loading of MCM2-7 onto DNA

Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
Abstract | Imperial College London News |