Tag - DNA replication

VACANCY: Research associate x 2 (MED01698)

Reference: MED01698
Date posted: 27 January 2020
Closing date: 26 February 2020

Two research associate positions are available to study new mechanisms in DNA replication and the epigenetic basis of chromatin structure.

Position 1:

The successful applicant should have expertise in protein biochemistry and structural biology and will employ in vitro reconstitution and cryo-electron microscopy to investigate either:

  1. Novel DNA replication mechanisms, or
  2. how epigenetics affect chromatin structure.

Position 2:

The successful applicant should have expertise in genomics and proteomics and will employ novel sequencing-based approaches together with proteomics to investigate either:

  1. Novel mechanisms in the initiation of DNA replication and DNA repair, or
  2. nucleosome/chromatin organisation and structure at a genome-wide level in order to understand gene regulation in health and disease.

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New paper proposes lagging-strand DNA extrusion mechanism

Noguchi, Y., Yuan, Z., Bai, L., Schneider, S., Zhao, G., Stillman, B., Speck, C., Li, H. (2017). Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proceedings of the National Academy of Sciences of the United States of America 114, E9529-E9538.
Abstract | Full text |

New paper shows Cdc6 ATPase activity promotes DNA replication

Chang, F., Riera, A., Evrin, C., Sun, J., Li, H., Speck, C., Weinreich, M. (2015). Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication. eLife 4, e05759.
Abstract | Full text | Biomedical Picture of the Day | MRC Clinical Sciences Centre News | Imperial College London News |

New paper on regulation of loading of MCM2-7 onto DNA

Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
Abstract | Imperial College London News |

Report on Keystone Symposium on ‘DNA Replication and Recombination’

A report of the Keystone Symposium ‘DNA Replication and Recombination’ held in Keystone, USA, 27 February to 4 March 2011.

Johansson, E., Speck, C. and Chabes, A. (2011). A top-down view on DNA replication and recombination from 9,000 feet above sea level. Genome Biology 12, 304.
| Full text |