Papers

Yuan, Z., Riera, A., Bai, L., Sun, J., Nandi, S., Spanos, C., Chen, Z. A., Barbon, M., Rappsilber, J., Stillman, B., Speck, C., Li, H. (2017). Structural basis of Mcm2–7 replicative helicase loading by ORC–Cdc6 and Cdt1. Nature Structure & Molecular Biology 24, 316-324.
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Speck, C. (2016). Exceeding the limits – Cdc45 overexpression turns bad. Cell Cycle 15, 1809-1810.
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Tognetti, S., Speck, C. (2016). Replicating repetitive DNA. Nature Cell Biology 18, 593-594.
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Chang, F., Riera, A., Evrin, C., Sun, J., Li, H., Speck, C., Weinreich, M. (2015). Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication. eLife 4, e05759.
| Abstract | Full text | Biomedical Picture of the Day | MRC Clinical Sciences Centre News | Imperial College London News

Herrera, M. C.*, Tognetti, S.*, Riera, A., Zech, J., Clarke, P., Fernández-Cid, A., Speck, C. (2015). A reconstituted system reveals how activating and inhibitory interactions control DDK dependent assembly of the eukaryotic replicative helicase. Nucleic Acids Research 43, 10238-10250.
*Shared first authorship
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Riera, A., Speck, C. (2015). MCM2-7 – Opening the gate to DNA Replication. Cell Cycle 14, 6-8.
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Tognetti, S., Riera, A., Speck, C. (2015) Switch on the engine: how the eukaryotic replicative MCM2–7 helicase becomes activated. Chromosoma 124, 13-26.
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Evrin, C.*, Fernández-Cid, A.*, Riera, A., Zech, J., Clarke, P., Herrera, M. C., Tognetti, S., Lurz, R., Speck, C. (2014). The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during pre-replicative complex formation. Nucleic Acids Research 42, 2257-2269.
*Shared first authorship
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Riera, A., Tognetti, S., Speck, C. (2014). Helicase loading: How to build a MCM2-7 double-hexamer. Seminars in Cell & Developmental Biology 30C, 104-109.
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Samel, S. A., Fernández-Cid, A., Sun, J., Riera, A., Tognetti, S., Herrera, C., Li, H., Speck, C. (2014). A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2–7 onto DNA. Genes & Development 28, 1653-1666.
| Abstract | Imperial College London News |

Silva, N., Ferrandiz, N., Barroso, C., Tognetti, S., Lightfoot, J., Telecan, O., Encheva, V., Faull, P., Hanni, S., Furger, A., Snidjers, A. P., Speck, C., Martinez-Perez, E. (2014). The fidelity of synaptonemal complex assembly is regulated by a signaling mechanism that controls early meiotic progression. Developmental Cell 31, 503-511.
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Sun, J.*, Fernandez-Cid, A.*, Riera, A.*, Tognetti, S., Yuan, Z., Stillman, B.†, Speck, C.†, Li, H.† (2014). Structural and mechanistic insights into Mcm2–7 double-hexamer assembly and function. Genes & Development 28, 2291-2303.
*Shared first authorship, †Corresponding authors
| Abstract | Biomedical Picture of the Day |

Evrin, C., Fernández-Cid, A., Zech, J., Herrera, M. C., Riera, A., Clarke, P., Brill, S., Lurz, R., Speck, C. (2013). In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2–7 hexamer dimerization. Nucleic Acids Research 41, 3162-3172.
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Fernández-Cid, A.*, Riera, A.*, Tognetti, S., Herrera, M. C., Samel, S., Evrin, C., Winkler, C., Gardenal, E., Uhle. S., Speck, C. (2013). An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer formation. Molecular Cell 50, 577-588.
*Shared first authorship
| Abstract | Full text | Molecular Cell Preview |

Riera, A., Fernández-Cid, A., Speck, C. (2013). The ORC/Cdc6/MCM2–7 complex, a new power player for regulated helicase loading. Cell Cycle 12, 2155-2156.
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Riera, A., Li, H., Speck, C. (2013). Seeing is believing: The MCM2–7 helicase trapped in complex with its DNA loader. Cell Cycle 12, 2917-2918.
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Sun, J.*, Evrin, C.*, Samel, S. A., Fernández-Cid, A., Riera, A., Kawakami, H., Stillman, B.†, Speck, C.†, Li, H.† (2013). Cryo-EM structure of a helicase loading intermediate containing ORC–Cdc6–Cdt1–MCM2-7 bound to DNA. Nature Structural & Molecular Biology 20, 944-951.
*Shared first authorship; †Corresponding authors
| Abstract | Full text | Brookhaven National Laboratory Newsroom |

Sun, J., Kawakami, H., Zech, J., Speck, C., Stillman, B., Li, H. (2012). Cdc6-induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopy. Structure 20, 534-544.
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Johansson, E., Speck, C. and Chabes, A. (2011). A top-down view on DNA replication and recombination from 9,000 feet above sea level. Genome Biology 12, 304.
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Evrin, C., Clarke, P., Zech, J., Lurz, R., Sun, J., Uhle, S., Li, H., Stillman, B., Speck, C. (2009). A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA. Proceedings of the National Academy of Sciences of the United States of America 106, 20240-20245.
| Abstract | Full text | F1000Prime |

Chen, Z.*, Speck, C.*, Wendel, P., Tang, C., Stillman, B., Li, H. (2008). The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America 105, 10326-10331.
*Contributed equally to work
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Majka, J., Speck, C. (2007). Analysis of protein-DNA interactions using surface plasmon resonance. Advances in Biochemical Engineering/Biotechnology 104, 13-36.
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Speck, C., Stillman, B. (2007). Cdc6 ATPase activity regulates ORC-Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication. The Journal of Biological Chemistry 282, 11705-11714.
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Speck, C., Chen, Z., Li, H., Stillman, B. (2005). ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA. Nature Structural & Molecular Biology 12, 965-971.
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de la Hoz, A. B., Pratto, F., Misselwitz, R., Speck, C., Weihofen, W., Welfle, K., Saenger, W., Welfle, H., Alonso, J. C. (2004). Recognition of DNA by ω protein from the broad-host range Streptococcus pyogenes plasmid pSM19035: analysis of binding to operator DNA with one to four heptad repeats. Nucleic Acids Research 32, 3136-3147.

Berenstein, D., Olesen, K., Speck, C. , Skovgaard, O. (2002). Genetic organization of the Vibrio harveyi dnaA gene region and analysis of the function of the V. harveyi DnaA protein in Escherichia coli. Journal of Bacteriology 184, 2533-2538.
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Pieper, U., Groll, D. H., Wünsch, S., Gast, F.-U., Speck, C., Mücke, N., Pingoud, A. (2002). The GTP-dependent restriction enzyme McrBC from Escherichia coli forms high-molecular mass complexes with DNA and produces a cleavage pattern with a characteristic 10-base pair repeat. Biochemistry 41, 5245-5254.
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Messer, W., Blaesing, F., Jakimowicz, D., Krause, M., Majka, J., Nardmann, J., Schaper, S., Seitz, H., Speck, C., Weigel, C., Wegrzyn, G., Welzeck, M., Zakrzewska-Czerwinska, J. (2001). Bacterial replication initiator DnaA. Rules for DnaA binding and roles of DnaA in origin unwinding and helicase loading. Biochimie 83, 5-12.
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Speck, C., Messer, W. (2001). Mechanism of origin unwinding: sequential binding of DnaA to double- and single-stranded DNA. The EMBO Journal 20, 1469-1476.
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Schaper, S., Nardmann, J., Lüder, G., Lurz, R., Speck, C., Messer, W. (2000). Identification of the chromosomal replication origin from Thermus thermophilus and its interaction with the replication initiator DnaA. Journal of Molecular Biology 299, 655-665.
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Skarstad, K., Lueder, G., Lurz, R., Speck, C., Messer, W. (2000). The Escherichia coli SeqA protein binds specifically and co-operatively to two sites in hemimethylated and fully methylated oriC. Molecular Microbiology 36, 1319-1326.
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Köhler, M., Speck, C., Christiansen, M., Bischoff, F. R., Prehn, S., Haller, H., Görlich, D., Hartmann, E. (1999). Evidence for distinct substrate specificities of importin α-family members in nuclear protein import. Molecular and Cellular Biology 19, 7782-7791.
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Messer, W., Blaesing, F., Majka, J., Nardmann, J., Schaper, S., Schmidt, A., Seitz, H., Speck, C., Tüngler, D., Wegrzyn, G., Weigel, C., Welzeck, M., Zakrzewska-Czerwinska, J. (1999). Functional domains of DnaA proteins. Biochimie 81, 819-825.
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Speck, C., Weigel, C., Messer, W. (1999). ATP- and ADP-DnaA protein, a molecular switch in gene regulation. The EMBO Journal 18, 6169-6176.
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Jakimowicz, D., Majka, J., Messer, W., Speck, C., Fernandez, M., Martin, M. C., Sanchez, J., Schauwecker, F., Keller, U., Schrempf, H., Zakrzewska-Czerwinska, J. (1998). Structural elements of the Streptomyces oriC region and their interactions with the DnaA protein. Microbiology 144, 1281-1290.
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Szalewska-Palasz, A., Weigel, C., Speck, C., Srutkowska, S., Konopa, G., Lurz, R., Marszalek, J., Taylor, K., Messer, W., Wegrzyn, G. (1998). Interaction of the Escherichia coli DnaA protein with bacteriophage λ DNA. Molecular and General Genetics 259, 679-688.
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Speck, C., Weigel, C., Messer, W. (1997). From footprint to toeprint: a close-up of the DnaA box, the binding site for the bacterial initiator protein DnaA. Nucleic Acids Research 5, 3242-3247.
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